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empty pgl3-basic plasmid  (Promega)

 
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    Promega empty pgl3-basic plasmid
    Empty Pgl3 Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+pgl3+basic+plasmid/pgl3+basic/pm36403704-86-19-42
    Average 90 stars, based on 1 article reviews
    empty pgl3-basic plasmid - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cotransfection:

    Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
    Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..

    Plasmid Preparation:

    Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
    Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..

    Negative Control:

    Article Title: Regulation of Cytochrome P450 2a5 by Artemisia capillaris and 6,7-Dimethylesculetin in Mouse Hepatocytes
    Article Snippet: As a positive control, hepatocytes were co-transfected with pGL3 control plasmid (Promega, Madison, WI, United States) (500 ng/well) which constitutively expresses the luciferase gene. .. Co-transfection with the empty pGL3 basic plasmid (Promega, Madison, WI, United States) (500 ng/well) served as a negative control. ..



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    Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Journal: Genes

    Article Title: Functional Analysis of Promoter Variants in Genes Involved in Sex Steroid Action, DNA Repair and Cell Cycle Control

    doi: 10.3390/genes10030186

    Figure Lengend Snippet: Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Article Snippet: Similar experiments were performed with the empty promoterless pGL3-Basic plasmid (Promega) and the SV40-driven Firefly Luciferase pGL3-control plasmid (Promega, Madison, WI, USA) as negative and positive controls, respectively.

    Techniques: Activity Assay, Luciferase, Plasmid Preparation, Clone Assay, Control, Expressing